Applications are invited for a Postdoctoral position in the Photosymbiosis team of the Cell and Plant Physiology Laboratory (IRIG/UMR 5168 – CNRS -CEA – Univ. Grenoble Alpes – INRAe) (Grenoble, France) in collaboration with the Structural Biology Institute (IBS, Grenoble). We are seeking a highly motivated and enthusiastic scientist with excellent skills and knowledge in 3D electron microscopy (sample preparation for cryo-imaging and/or image processing). The project is part of the SymbioChip project funded by the Gordon and Betty Moore Foundation and led by the Roscoff Marine Station (CNRS). The candidate will closely collaborate with consortium members from the Roscoff Marine Station, the Bigelow Laboratory for Ocean Sciences (USA) and the Friedrich-Schiller University Jena (Germany). The Gordon and Betty Moore Foundation’s Symbiosis in Aquatic Systems initiative is investing over the next three years to support 42 international teams of scientists working collaboratively to develop tools and methods to advance model systems in aquatic symbiosis. The Initiative’s funding aims to equip the scientific community with infrastructure such as new genetic tools, cultivation methods, and nanoscale microscopy to improve experimental capabilities in aquatic symbiosis research over the coming decade (https://tinyurl.com/y3t8ajbw).
Project: The main objective of the project is to develop 3D imaging in “cryo” mode on single-celled photosymbioses collected in the ocean (symbiosis between a host cell and intracellular microalgae). More specifically, the aim of the candidate will be to develop and apply a sample preparation workflow with correlative microscopy, and cryo 3D imaging (FIB-SEM and cryo-TEM) to unveil the architecture of the symbiotic cells at high resolution. The project will also involve sampling campaigns in the Mediterranean Sea.
– Implementation of a workflow in cryo mode for 3D electron microscopy
– Image analysis (filtration, segmentation, 3D reconstruction)
– Cryo-fluorescence microscopy
– Communication through international meetings and workshops.
Competences and qualifications:
– PhD in a relevant biological subject (e.g. cellular biology).
– The candidate should have a good academic track record, and solid background and research experience in 3D electron microscopy
– Knowledge and expertise in sample preparation for 3D electron microscopy (FIB-SEM, cryo-TEM). Expertise in cryo FIB-SEM will be appreciated.
– OR (not required) Knowledge and expertise in image analysis for 3D reconstruction after FIB-SEM or cryo-TEM.
– Excellent written and oral communication skills and proficiency in English are required. French is optional.
– The candidate should have good interpersonal skills and ability to work independently, but also to interact well within an international research group.